Preparation of methyl esters

Preparation of fatty acid methyl esters for gas-liquid chromatography.

A convenient method using commercial aqueous concentrated HCl (conc. HCl; 35%, w/w) as an acid catalyst was developed for preparation of fatty acid methyl esters (FAMEs) from sterol esters, triacylglycerols, phospholipids, and FFAs for gas-liquid chromatography (GC). An 8% (w/v) solution of HCl in methanol/water (85:15, v/v) was prepared by diluting 9.7 ml of conc. HCl with 41.5 ml of methanol....

Ccxlviii. the Preparation of Methyl Esters of Pectic Acid. by Harold William Buston and Homi

Preparation and synthetic applications of 2-halotryptophan methyl esters: synthesis of spirotryprostatin B.

Preparation of Fatty Acid Methyl Esters and Dimethylacetals from Lipids with Boron Fluoride--methanol.

Fatty acid methyl esters and dimethylacetals suitable for gas chromatographic analysis were prepared by treatment of lipids with boron fluoride-methanol (140 g BFI per liter of methanol). This reagent is stable and easy to handle. Reaction conditions were investigated for triglycerides, diglycerides, monoglycerides, free fatty acids, sterol esters, phosphatidyl ethanolamines, phosphatidyl serin...

Improving the Analysis of Fatty Acid Methyl Esters Using Automated Sample Preparation Techniques

An automated method for esterifying fatty acids in canola oil samples is presented. Using the Agilent 7696A Sample Prep WorkBench, a side-by-side comparison was undertaken comparing a manual method employing automatic pipettors to a method developed for this automated system. Using the Agilent 7696A Sample Prep WorkBench, preparation of 10 samples resulted in 3% RSD for both an acid-catalyzed a...